FLNC ELISA Kit (Human) (OKCD08737)

Catalog No: OKCD08737

Size:96WELLS

FLNC ELISA Kit (Human) (OKCD08737) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin C Gamma (FLNC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin C Gamma (FLNC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin C Gamma (FLNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Filamin C Gamma (FLNC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin C Gamma (FLNC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin C Gamma (FLNC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FLNC Microplate: 96 Wells (12 x 8 Well strips)
  • FLNC Lyophilized Standard: 2
  • 100X FLNC HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.126ng/mL

Target Info

• Gene Symbol: FLNC
• Gene Full Name: filamin C
• Alias Symbols: ABP-280;ABP280A;ABP-280-like protein;ABPA;ABPL;ABP-L;ABP-L, gamma filamin;actin binding protein 280;Actin-binding-like protein;CMH26;filamin C, gamma;filamin-2;filamin-C;FLN2;gamma filamin;Gamma-filamin;MFM5;MPD4;RCM5.
• NCBI Gene Id: 2318
• Protein Name: Filamin-C
• Description of Target: Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers.
• Uniprot ID: Q14315
• Protein Accession #: NP_001120959.1
• Nucleotide Accession #: NM_001127487.1