FLNB ELISA Kit (Human) (OKCD08736)

Catalog No: OKCD08736

Size:96WELLS

FLNB ELISA Kit (Human) (OKCD08736) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin B Beta (FLNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin B Beta (FLNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin B Beta (FLNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Filamin B Beta (FLNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin B Beta (FLNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin B Beta (FLNb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FLNB Microplate: 96 Wells (12 x 8 Well strips)
  • FLNB Lyophilized Standard: 2
  • 100X FLNB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 5.4pg/mL

Target Info

• Gene Symbol: FLNB
• Gene Full Name: filamin B
• Alias Symbols: ABP-278;ABP-280;ABP-280 homolog;actin binding protein 278;actin-binding-like protein;AOI;beta-filamin;FH1;filamin B, beta;filamin homolog 1;filamin-3;filamin-B;FLN1L;FLN-B;Larsen syndrome 1 (autosomal dominant);LRS1;SCT;TABP;TAP;thyroid autoantigen;Truncated actin-binding protein.
• NCBI Gene Id: 2317
• Protein Name: Filamin-B
• Description of Target: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
• Uniprot ID: O75369
• Protein Accession #: NP_001157789.1
• Nucleotide Accession #: NM_001164317.1