FLNA ELISA Kit (Human) (OKEH08137)

Catalog No: OKEH08137

Size:96T

Human FLNA ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	107-117% (n = 20)	105-118% (n = 20)	88-98% (n = 20)	105-117% (n = 20)
  EDTA plasma(n=5)	82-92% (n = 20)	88-97% (n = 20)	88-98% (n = 20)	95-106% (n = 20)
  heparin plasma(n=5)	112-120% (n = 20)	118-127% (n = 20)	103-113% (n = 20)	96-106% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology FLNA ELISA Kit (Human) (OKEH08137) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLNA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLNA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLNA captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.5% (n = 20)
  Mean Inter-assay CV%: <=6.9% (n = 20)
• ELISA Kit Component:
  • FLNA Microplate: 96 Wells (12 x 8 Well strips)
  • FLNA Lyophilized Standard: 2
  • 100X Biotinylated FLNA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance.
  Subcellular Location: Cytoplasm Cell cortex Cytoplasm Cytoskeleton
  Unigene: Hs.195464
  SMR: P21333
  String: 9606.ENSP00000358866
  KEGG: hsa:2316
  MIM: 300017
  Pfam: PF00307
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.056ng/mL
• Specificity: Natural and recombinant Human Filamin-A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FLNA
• Gene Full Name: filamin A
• Alias Symbols: ABP-280;ABPX;actin binding protein 280;Actin-binding protein 280;alpha-filamin;CSBS;CVD1;endothelial actin-binding protein;epididymis secretory sperm binding protein;FGS2;filamin A, alpha;filamin-1;filamin-A;FLN;FLN1;FLN-A;FMD;MNS;NHBP;non-muscle filamin;OPD;OPD1;OPD2;XLVD;XMVD.
• NCBI Gene Id: 2316
• Protein Name: P21333
• Description of Target: Filamin-A
• Uniprot ID: https://www.uniprot.org/uniprot/P21333
• Protein Accession #: NP_001104026.1
• Nucleotide Accession #: NM_001110556.1