FLI1 Antibody (OAGA04152)

Catalog No: OAGA04152

Size:100 ul

FLI1 antibody detects FLI1 protein by western blot analysis.
A. 30 ug BCL-1 whole cell lysate/extract
10% SDS-PAGE
FLI1 antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

FLI1 antibody detects FLI1 protein by western blot analysis.
A. 50 ug rat kidney lysate/extract
10% SDS-PAGE
FLI1 antibody dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1 antibody at 1:500 dilution.

FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FLI1 protein stained by FLI1 antibody diluted at 1:500.
Blue: Hoechst 33343 staining.

FLI1 antibody immunoprecipitates FLI1 protein in IP experiments.
IP samples: Raji whole cell extract
A. 40 ug Raji whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 antibody
10 % SDS-PAGE
The immunoprecipitated FLI1 protein was detected by FLI1 antibody diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.95 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: FLI1
• Gene Full Name: Fli-1 proto-oncogene, ETS transcription factor
• Alias Symbols: EWSR2, SIC-1, BDPLT21
• NCBI Gene Id: 2313
• Protein Name: Friend leukemia integration 1 transcription factor
• Description of Target: This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q01543
• Protein Accession #: NP_001161153.1
• Nucleotide Accession #: NM_001167681.2