FLI1 Antibody - N-terminal region (OABB01342)

Catalog No: OABB01342

Size:100UG

Anti-FLI1 antibody, OABB01342, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate

Anti-FLI1 antibody, OABB01342, IHC(P)
IHC(P): Human Tonsil Tissue

Anti-FLI1 antibody, OABB01342, IHC(P)
IHC(P): Rat Spleen Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Chicken, Human, Mouse, Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry, Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1 (54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: FLI1
• Gene Full Name: Fli-1 proto-oncogene, ETS transcription factor
• Alias Symbols: BDPLT21;Ewing sarcoma breakpoint region 2;EWSR2;Friend leukemia integration 1 transcription factor;Friend leukemia virus integration 1;Proto-oncogene Fli-1;SIC-1;transcription factor ERGB.
• NCBI Gene Id: 2313
• Protein Name: Q01543
• Description of Target: Friend leukemia integration 1 transcription factor
• Uniprot ID: https://www.uniprot.org/uniprot/Q01543
• Molecular Weight: 50982 MW