Catalog No: OKCD02558
Size:96WELLS
Price: $700.00
SKU
OKCD02558
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Datasheets/ManualsPrintable datasheet for FLG ELISA Kit (Mouse) (OKCD02558)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filaggrin (FLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filaggrin (FLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filaggrin (FLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Filaggrin (FLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filaggrin (FLG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filaggrin (FLG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-FLG Microplate96 Wells (12 x 8 Well strips)
FLG Lyophilized Standard2
100X Biotinylated FLG Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.114 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Filaggrin (FLG). No significant cross-reactivity or interference between Filaggrin (FLG) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolFLG
Alias SymbolsAW107830, Filaggrin, fillagrin, ft, profilaggrin
Protein NameFilaggrin
Description of TargetAggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Uniprot IDP11088
Protein Size (# AA)336
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