FKBP52 Antibody : Biotin (OASE00057-Biotin)

Catalog No: OASE00057-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Canine, Hamster
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: Hi52C
• Isotype: IgG
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IP, IHC
• Additional Information: Background Info: Detects an ~52kDa protein representing FKBP52 in cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS PAGE.
  Scientific Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2).
  FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3).
  The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3).
  FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
  Certificate of Analysis: 0.5ug/ml was sufficient for detection of FKBP52 in 20ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP52
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:2000 (WB), 5ug with 10-20uL Protein A beads (IP), 1:250 (IHC)
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394.
  2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002)
  J Biol. Chem. 277 (7): 4597-4600.
  3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA.
  101(22): 8348-8353.
  4. Denny W.B., Prapapanich V., Smith D.F., and
  Scammell J.G. (2005) Endocrinology 146(7):3194-3201.
  5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.

Target Info

• Gene Symbol: FKBP4
• Gene Full Name: FK506 binding protein 4, 59kDa
• Alias Symbols: HBI, p52, Hsp56, FKBP51, FKBP52, FKBP59, PPIase
• NCBI Gene Id: 2288
• Uniprot ID: Q02790
• Protein Accession #: NP_002005.1