Catalog No: OKEH00685
Size:96T
Price: $525.00
SKU
OKEH00685
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FKBP5 ELISA Kit (Human) (OKEH00685) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FKBP5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FKBP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FKBP5 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-FKBP5 Microplate96 Wells (12 x 8 Well strips)
FKBP5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FKBP5 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP5
Publications

Glucocorticoid receptor mutations and clinical sensitivity to glucocorticoid in Chinese multiple sclerosis patients. Neurol Sci. 41, 2767-2771 (2020). 32277392

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolFKBP5
Gene Full NameFKBP prolyl isomerase 5
Alias Symbols51 kDa FK506-binding protein;51 kDa FKBP;54 kDa progesterone receptor-associated immunophilin;AIG6;androgen-regulated protein 6;FF1 antigen;FK506 binding protein 5;FK506-binding protein 5;FKBP51;FKBP-51;FKBP54;HSP90-binding immunophilin;P54;peptidylprolyl cis-trans isomerase;peptidyl-prolyl cis-trans isomerase FKBP5;PPIase;PPIase FKBP5;Ptg-10;rotamase;T-cell FK506-binding protein.
NCBI Gene Id2289
Protein NameQ13451
Description of TargetPeptidyl-prolyl cis-trans isomerase FKBP5
Uniprot IDhttps://www.uniprot.org/uniprot/Q13451
Protein Accession #NP_001139247.1
Nucleotide Accession #NM_001145775.2
Protein Size (# AA)457
Molecular Weight51 kDa
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