FKBP4 ELISA Kit (Human) (OKEH07704)

Catalog No: OKEH07704

Size:96T

Human FKBP4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	81-91% (n = 20)	108-118% (n = 20)	80-90% (n = 20)	111-120% (n = 20)
  EDTA plasma(n=5)	97-107% (n = 20)	95-105% (n = 20)	98-109% (n = 20)	97-97% (n = 20)
  heparin plasma(n=5)	109-118% (n = 20)	96-107% (n = 20)	94-105% (n = 20)	101-111% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology FKBP4 ELISA Kit (Human) (OKEH07704) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FKBP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FKBP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FKBP4 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.0% (n = 20)
  Mean Inter-assay CV%: <=8.1% (n = 20)
• ELISA Kit Component:
  • FKBP4 Microplate: 96 Wells (12 x 8 Well strips)
  • FKBP4 Lyophilized Standard: 2
  • 100X Biotinylated FKBP4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
  Subcellular Location: Cytoplasm Cytosol Mitochondrion Nucleus Cytoplasm Cytoskeleton Cell projection Axon Shuttles from mitochondria to nucleus; co-localizes in mitochondria with the glucocorticoid receptor (PubMed:21730050). Colocalized with MAPT/TAU in the distal part of the primary cortical neurons (By similarity).
  Unigene: Hs.524183
  SMR: Q02790
  String: 9606.ENSP00000001008
  KEGG: hsa:2288
  MIM: 600611
  Pfam: PF00254
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.129ng/mL
• Specificity: Natural and recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FKBP4
• Gene Full Name: FKBP prolyl isomerase 4
• Alias Symbols: 51 kDa FK506-binding protein;52 kDa FK506-binding protein;59 kDa immunophilin;FK506 binding protein 4, 59kDa;FK506-binding protein 4;FKBP51;FKBP52;FKBP59;HBI;HSP binding immunophilin;Hsp56;HSP-binding immunophilin;Immunophilin FKBP52;p52;peptidylprolyl cis-trans isomerase;peptidyl-prolyl cis-trans isomerase FKBP4;PPIase;rotamase;T-cell FK506-binding protein, 59kD.
• NCBI Gene Id: 2288
• Protein Name: Q02790
• Description of Target: Peptidyl-prolyl cis-trans isomerase FKBP4
• Uniprot ID: https://www.uniprot.org/uniprot/Q02790
• Protein Accession #: NP_002005.1
• Nucleotide Accession #: NM_002014.3