FKBP4 ELISA Kit (Human) (OKCD00439)

Catalog No: OKCD00439 (Formerly GWB-KBBFW2)

Size:96WELLS

Human FKBP4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 4 (FKBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 4 (FKBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 4 (FKBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FK506 Binding Protein 4 (FKBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FKBP4 Microplate: 96 Wells (12 x 8 Well Strips)
  • FKBP4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated FKBP4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 4 (FKBP4). No significant cross-reactivity or interference between FK506 Binding Protein 4 (FKBP4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FKBP4
• Gene Full Name: FKBP prolyl isomerase 4
• Alias Symbols: 51 kDa FK506-binding protein;52 kDa FK506-binding protein;59 kDa immunophilin;FK506 binding protein 4, 59kDa;FK506-binding protein 4;FKBP51;FKBP52;FKBP59;HBI;HSP binding immunophilin;Hsp56;HSP-binding immunophilin;Immunophilin FKBP52;p52;peptidylprolyl cis-trans isomerase;peptidyl-prolyl cis-trans isomerase FKBP4;PPIase;rotamase;T-cell FK506-binding protein, 59kD.
• NCBI Gene Id: 2288
• Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Description of Target: Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
• Uniprot ID: Q02790
• Protein Accession #: NP_002005
• Nucleotide Accession #: NM_002014.3
• Protein Size (# AA): 459