FKBP4 Antibody (OACA07193)

Catalog No: OACA07193

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

All lanes: Peptidyl-prolyl cis-trans isomerase FKBP4 antibody at 1ug/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 52kDa
Observed band size: 52kDa

Immunoprecipitating FKBP4 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA03031 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA03031(4ug)+ MCF-7 whole cell lysate(500ug)
Lane 3: MCF-7 whole cell lysate (20ug)

All lanes: Peptidyl-prolyl cis-trans isomerase FKBP4 antibody at 1ug/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 52kDa
Observed band size: 52kDa

Immunoprecipitating FKBP4 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA03031 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA03031(4ug)+ MCF-7 whole cell lysate(500ug)
Lane 3: MCF-7 whole cell lysate (20ug)

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP4 protein (220-459AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex." Wu B., Li P., Liu Y., Lou Z., Ding Y., Shu C., Ye S., Bartlam M., Shen B., Rao Z. Proc. Natl. Acad. Sci. U.S.A. 101:8348-8353(2004). [2]"Structure of the N-terminal domain of human FKBP52."Li P., Ding Y., Wu B., Shu C., Shen B., Rao Z.Acta Crystallogr. D 59:16-22(2003). [3]"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014).

Target Info

• Gene Symbol: FKBP4
• Gene Full Name: FKBP prolyl isomerase 4
• Alias Symbols: 51 kDa FK506-binding protein;52 kDa FK506-binding protein;59 kDa immunophilin;FK506 binding protein 4, 59kDa;FK506-binding protein 4;FKBP51;FKBP52;FKBP59;HBI;HSP binding immunophilin;Hsp56;HSP-binding immunophilin;Immunophilin FKBP52;p52;peptidylprolyl cis-trans isomerase;peptidyl-prolyl cis-trans isomerase FKBP4;PPIase;rotamase;T-cell FK506-binding protein, 59kD.
• NCBI Gene Id: 2288
• Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Description of Target: Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
• Uniprot ID: Q02790
• Protein Accession #: NP_002005
• Nucleotide Accession #: NM_002014