Fibronectin Auto-Antibody (Human) ELISA Kit (OKCA00458)

Catalog No: OKCA00458

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Fibronectin Auto-Antibody (Human) ELISA Kit (OKCA00458) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Fibronectin antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Fibronectin Auto-Antibody captured in well.
• ELISA Kit Reproducibility: Three different samples of known Fibronectin Auto-Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • Fibronectin Auto-Antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Negative Control: 1 x 800 ul
  • Positive Control: 1 x 800 ul
  • 100X Anti-Human Antibody HRP-conjugate: 1 x 120 ul
  • HRP-conjugate Diluent: 1 x 20 ml
  • Sample Diluent: 2 x 20 ml
  • 25X Wash Buffer: 1 x 20 ml
  • TMB Substrate: 1 x 10 ml
  • Stop Solution: 1 x 10 ml
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum and Plasma
• Assay Info: Assay Methodology: Qualitative Reverse Capture ELISA

Target Info

• Alias Symbols: FN
• Protein Name: Fibronectin Auto-Antibody
• Description of Target: Fibronectin Auto-Antibody