Fibrinogen ELISA Kit (Rat) (OKIA00143)

Catalog No: OKIA00143 (formerly GWB-8088AE)

Size:96 Wells

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Rat
• Product Format: In Solution
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: Aviva Systems Biology Fibrinogen ELISA Kit (Rat) (OKIA00143) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fibrinogen alpha chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fibrinogen alpha chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fibrinogen alpha chain captured in the well.
• ELISA Kit Range: 25 ng/ml - 800 ng/ml
• ELISA Kit Component:
  • Anti-Rat Fibrinogen Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Rat Fibrinogen Calibrator: 1
• Additional Information: Intended Use: The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of rats.
  Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Rat Fibrinogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-RAT FIBRINOGEN ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat FIB.
  7. RAT FIBRINOGEN CALIBRATOR
  One vial containing Rat Fibrinogen calibrator.
  
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 3.098 ng/ml
• Predicted Homology Based on Immunogen Sequence: Rat
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Alias Symbols: Fibrinogen alpha chain, FGA
• Protein Name: Fibrinogen alpha chain
• Description of Target: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
• Uniprot ID: P06399