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Catalog No: OKIA00117 (Formerly GWB-298CF7)
Size:1x96WELLPLATE
Price: $500.00
SKU
OKIA00117
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration70 Minutes
ELISA Kit PrincipleAviva Systems Biology Fibrinogen ELISA Kit (Pig) (OKIA00117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fibrinogen alpha chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fibrinogen alpha chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fibrinogen alpha chain captured in the well.
ELISA Kit Range12.5 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Pig Fibrinogen Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Pig Fibrinogen Calibrator1
Additional InformationIntended Use: The Fibrinogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of Pigs.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Pig Fibrinogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-PIG FIBRINOGEN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Pig FIB.
7. PIG FIBRINOGEN CALIBRATOR
One vial containing a Pig Fibrinogen Calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.713 ng/ml
Predicted Homology Based on Immunogen SequencePig
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsFibrinogen alpha chain, FGA
Protein NameFibrinogen alpha chain
Description of TargetCleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Uniprot IDP14460
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