FHL2 Antibody - C-terminal region (OAGA03019)

Catalog No: OAGA03019

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FHL2 antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL2 antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FHL2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: FHL2
• Gene Full Name: four and a half LIM domains 2
• Alias Symbols: DRAL, AAG11, FHL-2, SLIM3, SLIM-3
• NCBI Gene Id: 2274
• Protein Name: four and a half LIM domains protein 2
• Description of Target: This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.
• Uniprot ID: Q14192
• Protein Accession #: NP_001034581.1
• Nucleotide Accession #: NM_001039492.2