FHL1 Antibody - C-terminal region : HRP (ARP34378_T100-HRP)

Catalog No: ARP34378_T100-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FHL1
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 100%
• Peptide Sequence: Synthetic peptide located within the following region: YYCVDCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCFHCKKCS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-FHL1 (ARP34378_T100-HRP) antibody is Catalog # AAP34378 (Previous Catalog # AAPY00220)
• Reference: McGrath,M.J., et al., (2003) Am. J. Physiol., Cell Physiol. 285 (6), C1513-C1526
• Publications:

  Windpassinger, C. et al. An X-linked myopathy with postural muscle atrophy and generalized hypertrophy, termed XMPMA, is caused by mutations in FHL1. Am. J. Hum. Genet. 82, 88-99 (2008). WB, IHC, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 18179888

  Quinzii, C. M. et al. X-linked dominant scapuloperoneal myopathy is due to a mutation in the gene encoding four-and-a-half-LIM protein 1. Am. J. Hum. Genet. 82, 208-13 (2008). WB, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 18179901

  Schoser, B. et al. Consequences of mutations within the C terminus of the FHL1 gene. Neurology 73, 543-51 (2009). WB, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 19687455

  Gueneau, L. et al. Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy. Am. J. Hum. Genet. 85, 338-53 (2009). IHC, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 19716112

  Sharma, P., Shathasivam, T., Ignatchenko, V., Kislinger, T. & Gramolini, A. O. Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis. Mol. Biosyst. 7, 1185-96 (2011). WB, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 21246116

  Dong, R. et al. Cells with dendritic cell morphology and immunophenotype, binuclear morphology, and immunosuppressive function in dendritic cell cultures. Cell. Immunol. 272, 1-10 (2011). WB, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 22053194

  Koike, K. et al. High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer. Int. J. Oncol. 42, 141-50 (2013). WB, IHC, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 23123766

  Fujii, T. et al. A case of adult-onset reducing body myopathy presenting a novel clinical feature, asymmetrical involvement of the sternocleidomastoid and trapezius muscles. J. Neurol. Sci. 343, 206-10 (2014). WB, Mouse, Human, Sheep, Dog, Bovine, Horse, Guinea pig, Rat, Rabbit 24928078

Target Info

• Gene Symbol: FHL1
• Gene Full Name: Four and a half LIM domains 1
• Alias Symbols: KYOT, SLIM, FCMSU, FHL-1, FHL1A, FHL1B, FLH1A, SLIM1, XMPMA, RBMX1A, RBMX1B, SLIM-1, SLIMMER
• NCBI Gene Id: 2273
• Protein Name: Four and a half LIM domains protein 1
• Description of Target: LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.
• Uniprot ID: Q6IB30
• Protein Accession #: NP_001440
• Nucleotide Accession #: NM_001449
• Protein Size (# AA): 280
• Molecular Weight: 32kDa
• Protein Interactions: SUMO2; UBC; SRPK1; SMAD4; SMAD3; SMAD2; LMNA; CSNK1D; NRIP1; ESR1; HIVEP3; SMURF1; UBE2E2; STAT4; RING1; RBPJ; FHL1; KCNA5; PRNP; USP15; HHV8GK18_gp81; SP1; TXNIP; DEAF1; PDE4DIP; AKAP12; EED; HES1; DBN1; CBX4; FHL2; SRF; MYBPC1;