FHIT Antibody (Phospho-Tyr114) (OAAF00584)

Catalog No: OAAF00584 (Formerly GWB-ASC024)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FHIT (Phospho-Tyr114) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FHIT around the phosphorylation site of Tyr114.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: FHIT (Phospho-Tyr114) antibody detects endogenous levels of FHIT only when phosphorylated at tyrosine 114.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Ohta M., Cell 84:587-597(1996).
  Druck T., Cancer Res. 57:504-512(1997).
  The MGC Project Team; Genome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: FHIT
• Gene Full Name: fragile histidine triad diadenosine triphosphatase
• Alias Symbols: AP3A hydrolase;AP3Aase;bis(5'-adenosyl)-triphosphatase;diadenosine 5',5'''-P1,P3-triphosphate hydrolase;dinucleosidetriphosphatase;FRA3B;Fragile histidine triad protein.
• NCBI Gene Id: 2272
• Protein Name: Bis(5'-adenosyl)-triphosphatase
• Description of Target: Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
• Uniprot ID: P49789
• Protein Accession #: NP_001159715.1
• Molecular Weight: 16 kDa