FGR Antibody (OAAF01519)

Catalog No: OAAF01519 (Formerly GWB-ASC890)

Size:100 ug

Western blot analysis of extracts from RAW264.7 cells, using FGR Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FGR.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: FGR (Ab-412) antibody detects endogenous levels of total FGR protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:40000
• Reference: Katamine S.,Mol. Cell. Biol. 8:259-266(1988).
  Gregory S.G.,Nature 441:315-321(2006).
  The MGC Project Team;Genome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: FGR
• Gene Full Name: FGR proto-oncogene, Src family tyrosine kinase
• Alias Symbols: c-fgr;c-fgr protooncogene;c-src2;c-src-2 proto-oncogene;feline Gardner-Rasheed sarcoma viral oncogene homolog;Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog;p55c-fgr;p55-c-fgr protein;p55-Fgr;p58c-fgr;p58-Fgr;proto-oncogene c-Fgr;proto-oncogene tyrosine-protein kinase FGR;SRC2;tyrosine-protein kinase Fgr;v-fgr feline Gardner-Rasheed sarcoma viral oncogene homolog.
• NCBI Gene Id: 2268
• Protein Name: Tyrosine-protein kinase Fgr
• Description of Target: Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.
• Uniprot ID: P09769
• Protein Accession #: NP_001036194.1
• Molecular Weight: 59 kDa