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Catalog No: OKCD02557
Size:96WELLS
Price: $625.00
SKU
OKCD02557
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Datasheets/ManualsPrintable datasheet for FGL2 ELISA Kit (Mouse) (OKCD02557)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)97-105%79-90%79-98%80-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Like Protein 2 (FGL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Like Protein 2 (FGL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Like Protein 2 (FGL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibrinogen Like Protein 2 (FGL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
sodium citrate plasma(n=5)82-9687
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Like Protein 2 (FGL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Like Protein 2 (FGL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-FGL2 Microplate96 Wells (12 x 8 Well strips)
FGL2 Lyophilized Standard2
100X Biotinylated FGL2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma,tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity0.135 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 2 (FGL2). No significant cross-reactivity or interference between Fibrinogen Like Protein 2 (FGL2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolFgl2
Gene Full Namefibrinogen-like protein 2
Alias SymbolsAI385601;associated with MHV-3 induced prothrombinase activity;cytotoxic T-lymphocyte-specific protein;Fibrinogen-like protein 2;fibroleukin;musfiblp;prothrombinase.
NCBI Gene Id14190
Protein NameFibroleukin
Description of TargetConverts prothrombin to thrombin.
Uniprot IDP12804
Protein Accession #NP_032039.2
Nucleotide Accession #NM_008013.4
Protein Size (# AA)432
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