FGG ELISA Kit (Bovine) (OKEH07264)

Catalog No: OKEH07264

Size:96T

Bovine FGG ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FGG ELISA Kit (Bovine) (OKEH07264) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGG captured in well.
• ELISA Kit Range: 7.8-500ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.9% (n = 20)
  Mean Inter-assay CV%: <=11.4% (n = 20)
• ELISA Kit Component:
  • FGG Microplate: 96 Wells (12 x 8 Well strips)
  • FGG Lyophilized Standard: 2
  • 100X Biotinylated FGG Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
  Subcellular Location: Secreted
  Unigene: Bt.107063
  SMR: P12799
  String: 9913.ENSBTAP00000008877
  KEGG: bta:280792
  Pfam: PF08702
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 3.8ng/ml
• Specificity: Natural and recombinant Bovine Fibrinogen gamma-B chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGG
• Gene Full Name: fibrinogen gamma chain
• Alias Symbols: fibrinogen gamma-B chain;fibrinogen, gamma polypeptide;gamma'.
• NCBI Gene Id: 280792
• Protein Name: P12799
• Description of Target: Fibrinogen gamma-B chain
• Uniprot ID: https://www.uniprot.org/uniprot/P12799
• Protein Accession #: NP_776336.1
• Nucleotide Accession #: NM_173911.2