Catalog No: OKEH00124
Size:96T
Price: $600.00
SKU
OKEH00124
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00124
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FGFR1 ELISA Kit (Human) (OKEH00124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGFR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGFR1 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
FGFR1 Microplate96 Wells (12 x 8 Well strips)
FGFR1 Lyophilized Standard2
100X Biotinylated FGFR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity12 pg/mL
SpecificityNatural and recombinant Human Fibroblast growth factor receptor 1
Publications

Validation of a Novel Modified Aptamer-Based Array Proteomic Platform in Patients with End-Stage Renal Disease. Diagnostics (Basel). 8, (2018). 30297602

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolFGFR1
Gene Full Namefibroblast growth factor receptor 1
Alias Symbolsbasic fibroblast growth factor receptor 1;BFGFR;bFGF-R-1;CD331;CEK;ECCL;FGFBR;FGFR-1;FGFR1/PLAG1 fusion;fibroblast growth factor receptor 1;FLG;FLT2;FLT-2;FMS-like tyrosine kinase 2;fms-related tyrosine kinase 2;HBGFR;heparin-binding growth factor receptor;HH2;HRTFDS;hydroxyaryl-protein kinase;KAL2;N-SAM;OGD;proto-oncogene c-Fgr.
NCBI Gene Id2260
Protein NameP11362
Description of TargetFibroblast growth factor receptor 1
Uniprot IDhttps://www.uniprot.org/uniprot/P11362
Protein Accession #NP_001167534
Nucleotide Accession #NM_001174063.1
Protein Size (# AA)822
Molecular Weight92 kDa
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