FGFR1 Antibody (OAAF00766)

Catalog No: OAAF00766 (Formerly GWB-ASC180)

Size:100 ug

Western blot analysis of extracts from 293 cells, using FGFR1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using FGFR1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid PBS (without Mg²⁺ and Ca²⁺) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FGFR1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: FGFR1 (Ab-654) antibody detects endogenous levels of total FGFR1 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000
  IF: 1:100~500
  ELISA: 1:1000
• Reference: Chitta S. Kasyapa, Blood, May 2006; 107: 3693 - 3699.
  Benoit Rousseau, Cancer Res., Apr 2004; 64: 2490 - 2495.
  Takayuki Nakayama, Blood, Feb 2007; 109: 1363 - 1372.
  Qing Wang, Development, Oct 2001; 128: 3867.

Target Info

• Gene Symbol: FGFR1
• Gene Full Name: fibroblast growth factor receptor 1
• Alias Symbols: basic fibroblast growth factor receptor 1;BFGFR;bFGF-R-1;CD331;CEK;ECCL;FGFBR;FGFR-1;FGFR1/PLAG1 fusion;fibroblast growth factor receptor 1;FLG;FLT2;FLT-2;FMS-like tyrosine kinase 2;fms-related tyrosine kinase 2;HBGFR;heparin-binding growth factor receptor;HH2;HRTFDS;hydroxyaryl-protein kinase;KAL2;N-SAM;OGD;proto-oncogene c-Fgr.
• NCBI Gene Id: 2260
• Protein Name: Fibroblast growth factor receptor 1
• Description of Target: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
• Uniprot ID: P11362
• Protein Accession #: NP_001167534.1
• Molecular Weight: 91 kDa