FGF9 Chemi-Luminescent ELISA Kit (Human) (OKCD03490)

Catalog No: OKCD03490

Size:96WELLS

Human FGF9 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	97-105%	82-91%	98-105%	79-92%
  EDTA Plasma (n=5)	82-103%	79-93%	78-93%	90-99%
  Heparin Plasma (n=5)	91-98%	96-105%	86-96%	78-91%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 9 (FGF9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 9 (FGF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 9 (FGF9) level in the sample or standard.
• ELISA Kit Range: 5.49-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-102	98
  EDTA Plasma (n=5)	80-99	87
  Heparin Plasma (n=5)	82-93	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 9 (FGF9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 9 (FGF9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FGF9 Microplate: 96 Wells (12 x 8 Well strips)
  • FGF9 Lyophilized Standard: 2
  • 100X FGF9 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.063 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF9
• Gene Full Name: fibroblast growth factor 9
• Alias Symbols: FGF-9;fibroblast growth factor 9;fibroblast growth factor 9 (glia-activating factor);GAF;Glia-activating factor;HBFG-9;HBGF-9;heparin-binding growth factor 9;SYNS3.
• NCBI Gene Id: 2254
• Protein Name: Fibroblast growth factor 9
• Description of Target: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
• Uniprot ID: P31371
• Protein Accession #: NP_002001
• Nucleotide Accession #: NM_002010