FGF3 Chemi-Luminescent ELISA Kit (Human) (OKCD03478)

Catalog No: OKCD03478

Size:96WELLS

Human FGF3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-92%	82-99%	82-101%	78-91%
  EDTA Plasma (n=5)	80-103%	87-96%	95-103%	78-94%
  Heparin Plasma (n=5)	91-105%	79-90%	82-97%	83-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 3 (FGF3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 3 (FGF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 3 (FGF3) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-103	101
  EDTA Plasma (n=5)	80-94	80
  Heparin Plasma (n=5)	88-95	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FGF3 Microplate: 96 Wells (12 x 8 Well strips)
  • FGF3 Lyophilized Standard: 2
  • 100X FGF3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.63 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF3
• Gene Full Name: fibroblast growth factor 3
• Alias Symbols: FGF-3;fibroblast growth factor 3;fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog);HBGF-3;heparin-binding growth factor 3;INT2;INT-2 proto-oncogene protein;murine mammary tumor virus integration site 2, mouse;oncogene INT2;proto-oncogene Int-2;V-INT2 murine mammary tumor virus integration site oncogene homolog.
• NCBI Gene Id: 2248
• Protein Name: Fibroblast growth factor 3
• Description of Target: Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal ear development.
• Uniprot ID: P11487
• Protein Accession #: NP_005238
• Nucleotide Accession #: NM_005247