Catalog No: OCOA09324
Size:20UG
Price: $179.00
SKU
OCOA09324
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

FGF21 Protein Lysate (OCOA09324)

Datasheets/ManualsPrintable datasheet for FGF21 Protein Lysate (OCOA09324)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFGF21 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFGF21
Gene Full Namefibroblast growth factor 21
Alias Symbolsfibroblast growth factor 21.
NCBI Gene Id26291
Protein NameNM_019113, NP_061986, Q9NSA1
Description of TargetFibroblast growth factor 21, fibroblast growth factor 21 precursor [Homo sapiens], Homo sapiens fibroblast growth factor 21 (FGF21), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NSA1
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_061986
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_019113
Molecular Weight19.4 kDa
Write Your Own Review
You're reviewing:FGF21 Protein Lysate (OCOA09324)
Your Rating