FGF2 ELISA Kit (Human) (OKBB00699)

Catalog No: OKBB00699

Size:96 wells

FGF2 ELISA Kit (Human) (OKBB00699) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human FGF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF2 has been precoated onto 96-well plates. Standards(E.coli,P143-S288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FGF2 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	150	752	1076	135	781	1071
  Standard deviation	10.95	54.89	79.62	10.8	62.48	92.1
  CV(%)	7.3	7.3	7.4	8	8	8.6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human FGF2 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human FGF2 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human FGF2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	150	132	141	131	138	7.69	5.5%
  Sample 2	752	641	642	667	675	45.37	6.7%
  Sample 3	1076	1179	1055	1199	1127	62.59	5.5%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
  O.D.	0.123	0.148	0.191	0.344	0.576	0.798	1.572	2.202

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human FGF2
• Immunogen: Expression system for standard: E.coli; Immunogen sequence:P143-S288
• Description: For quantitative detection of human FGF2 in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: FGF2
• Gene Full Name: fibroblast growth factor 2
• Alias Symbols: Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
• NCBI Gene Id: 2247
• Protein Name: Fibroblast growth factor 2
• Description of Target: FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
• Uniprot ID: P09038
• Protein Accession #: NP_001997.5
• Nucleotide Accession #: NM_002006.4