FGF2 ELISA Kit (Human) : 48 Wells (OKAA00030-48W)

Catalog No: OKAA00030-48W

Size:48 Wells

Standard curve of FGF2 protein using OKAA00030_48W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human FGF-basic Standard lyophilized, 500 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human FGF-basic antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human FGF-basic (8x12)
  2. 2 vials human FGF-basic Standard lyophilized, 1000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human FGF-basic monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 7 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human VEGF. The factors listed below were prepared at 200 ng/ml in Standard/ sample diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: EC-VEGF/PK1, PDGF-AA, PDGF-AB , PDGF-BB, PDGF
  Recombinant mouse: PDGF-2, VEGF120, VEGF164, VEGF R2(KDR)/Fc Chimera
  Recombinant Rat: VEGF 120(0.07%)
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF2
• Gene Full Name: fibroblast growth factor 2
• Alias Symbols: BFGF, FGFB, FGF-2, HBGF-2
• NCBI Gene Id: 2247
• Protein Name: Fibroblast growth factor 2
• Description of Target: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Uniprot ID: P09038
• Protein Accession #: NP_001997.5
• Nucleotide Accession #: NM_002006.4