FGF2 ELISA Kit (Bovine) (OKCA02038)

Catalog No: OKCA02038

Size:96 Wells

Bovine FGF2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bovine
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	92	87-98
  Serum (n=4)	1:2	97	93-102
  Serum (n=4)	1:4	84	81-89
  Serum (n=4)	1:8	88	83-95

• ELISA Kit Principle: Aviva Systems Biology FGF2 ELISA Kit (Bovine) (OKCA02038) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FGF2 captured in well.
• ELISA Kit Range: 6.25 pg/mL-400 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	101	98-104
  EDTA plasma (n=4)	92	89-97

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-FGF2 Microplate: 96 Wells (12 x 8 Well strips)
  • FGF2 Lyophilized Standard: 2 x 400 pg
  • 100X Biotinylated FGF2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.56 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF2
• Alias Symbols: BFGF, FGF-2, HBGF-2
• NCBI Gene Id: 281161
• Protein Name: Fibroblast growth factor 2
• Description of Target: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis.
• Uniprot ID: P03969
• Protein Accession #: NP_776481
• Nucleotide Accession #: NM_174056