FGF17 ELISA Kit (Human) (OKCA02101)

Catalog No: OKCA02101

Size:96 Wells

Human FGF17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology FGF17 ELISA Kit (Human) (OKCA02101) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGF17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FGF17 captured in well.
• ELISA Kit Range: 3.12 pg/mL -200 pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • FGF17 Microplate: 96 Wells (12 x 8 Well strips)
  • FGF17 Lyophilized Standard: 2 x 200 pg
  • 100X Biotinylated FGF17 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • (???)X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates And Tissue Homogenates
• Sensitivity: 0.78 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF17
• Alias Symbols: HH20, FGF-13, FGF-17
• NCBI Gene Id: 8822
• Protein Name: Fibroblast growth factor 17
• Description of Target: Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
• Uniprot ID: O60258
• Protein Accession #: NP_001291407
• Nucleotide Accession #: NM_001304478