Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02994
Size:96T
Price: $525.00
SKU
OKEH02994
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02994
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FGF15 ELISA Kit (Mouse) (OKEH02994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fgf15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf15 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Fgf15 Microplate96 Wells (12 x 8 Well strips)
Fgf15 Lyophilized Standard2
100X Biotinylated Fgf15 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39.4 pg/mL
SpecificityNatural and recombinant Mouse Fibroblast growth factor 15
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFgf15
Gene Full Namefibroblast growth factor 15
Alias SymbolsFGF-15;FGF19;Fgf8a;fibroblast growth factor 15;fibroblast growth factor 8a.
NCBI Gene Id14170
Protein NameO35622
Description of TargetFibroblast growth factor 15
Uniprot IDhttps://www.uniprot.org/uniprot/O35622
Protein Accession #NP_032029.1
Nucleotide Accession #NM_008003.2
Protein Size (# AA)218
Molecular Weight25 kDa
Write Your Own Review
You're reviewing:FGF15 ELISA Kit (Mouse) (OKEH02994)
Your Rating
We found other products you might like!