Catalog No: OKCD05284
Size:96WELLS
Price: $750.00
SKU
OKCD05284
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for FGF13 ELISA Kit (Human) (OKCD05284) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 13 (FGF13) and unlabeled High Sensitive Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 1.23-100pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.53pg/mL |
Gene Symbol | FGF13 |
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Gene Full Name | fibroblast growth factor 13 |
Alias Symbols | DEE90;FGF-13;FGF2;FHF2;FHF-2;fibroblast growth factor 13;fibroblast growth factor homologous factor 2;LINC00889. |
NCBI Gene Id | 2258 |
Protein Name | Fibroblast growth factor 13 |
Description of Target | Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus. |
Uniprot ID | Q92913 |
Protein Accession # | NP_001132970.1 |
Nucleotide Accession # | NM_001139498.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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