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Catalog No: OKCD03460
Size:96WELLS
Price: $850.00
SKU
OKCD03460
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FGF1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03460)

Datasheets/ManualsPrintable datasheet for FGF1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03460)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%96-105%78-101%94-101%
EDTA Plasma (n=5)88-95%83-103%95-102%98-105%
Heparin Plasma (n=5)82-97%89-98%97-105%80-98%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10197
EDTA Plasma (n=5)78-9590
Heparin Plasma (n=5)78-10186
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Fgf1 Microplate96 Wells (12 x 8 Well strips)
Fgf1 Lyophilized Standard2
100X Fgf1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity31.25 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFgf1
Gene Full Namefibroblast growth factor 1
Alias Symbolsacidic fibroblast growth factor;aFGF;Dffrx;Fam;Fgf;Fgf-1;Fgf2b;Fgfa;fibroblast growth factor 1;fibroblast growth factor 1 (acidic);fibroblast growth factor 2b;HBGF-1;heparin-binding growth factor 1.
NCBI Gene Id14164
Protein NameFibroblast growth factor 1
Description of TargetPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.
Uniprot IDP61148
Protein Accession #NP_034327
Nucleotide Accession #NM_010197
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