Catalog No: OPPA00483 (Formerly GWB-A1C65A)
Size:10UG
Price: $75.00
SKU
OPPA00483
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FGF 2 Protein (OPPA00483)

Datasheets/ManualsPrintable datasheet for OPPA00483
Product Info
Predicted Species ReactivityHuman
Product FormatLyophilized. PBS, pH 7.4 Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder
HostE. coli
Reconstitution and StorageReconstitution: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage: Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-b should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurificationThe FGF-b is purified by proprietary chromatographic techniques.
ConcentrationProtein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC
PurityGreater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological ActivityThe ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x106 Units/mg.
Peptide SequenceAAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Gene SymbolFGF 2
Alias SymbolsBFGF, FGFB, FGF-2, HBGF-2
NCBI Gene Id2247
Protein NameFibroblast growth factor 2
Description of TargetBasic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Uniprot IDP09038
Protein Accession #NP_001997.5
Protein Size (# AA)Recombinant
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