FGF 1 Protein (OPPA01509)

Catalog No: OPPA01509 (Formerly GWB-5F910D)

Size:10UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized at a concentration of 1 mg/ml in 5mM NaP, pH-7.5, 50mM NaCl. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.04 ng/ml.
  Product Introduction: Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
  Product Description: Fibroblast Growth Factor-acidic Mouse Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa.The FGF acidic is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-a should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Target Info

• Gene Symbol: mFGF 1
• Alias Symbols: Fam, Fgf, Fgfa, Dffrx, Fgf-1, Fgf2b
• NCBI Gene Id: 14164
• Protein Name: Fibroblast growth factor 1
• Description of Target: Recombinant Mouse Fibroblast Growth Factor-acidic
• Uniprot ID: P61148
• Protein Accession #: NP_034327.1
• Protein Size (# AA): Recombinant