FGF 1 Protein (OPPA00529)

Catalog No: OPPA00529 (Formerly GWB-54AEB0)

Size:10UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium phosphate pH=7.5 and 75mM NaCl. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18M Omega -cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.
  Biological Activity: The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is
  Product Introduction: Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
  Product Description: Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141amino acids and having a molecular mass of 15967 Dalton.The FGF acidic is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-a should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
• Purity: Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Target Info

• Gene Symbol: FGF 1
• Alias Symbols: AFGF, ECGF, FGFA, ECGFA, ECGFB, FGF-1, HBGF1, HBGF-1, GLIO703, ECGF-beta, FGF-alpha
• NCBI Gene Id: 2246
• Protein Name: Fibroblast growth factor 1
• Description of Target: Recombinant Human Fibroblast Growth Factor-acidic
• Uniprot ID: P05230
• Protein Accession #: NP_000791.1
• Protein Size (# AA): Recombinant