FGD4 Antibody (OAGA00800)

Catalog No: OAGA00800

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA00800 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SG xenograft, using FGD4(OAGA00800) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGD4(OAGA00800) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm, cytoskeleton (By similarity), Cell projection, filopodium (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 49 and 327 of FGD4 (Uniprot ID#Q96M96)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: FGD4
• Gene Full Name: FYVE, RhoGEF and PH domain-containing protein 4
• Alias Symbols: CMT4H, FRABP, ZFYVE6
• NCBI Gene Id: 121512
• Protein Name: FYVE, RhoGEF and PH domain-containing protein 4
• Uniprot ID: Q96M96
• Protein Accession #: NP_640334.2
• Molecular Weight: 87 kDa