FGD1 Antibody - N-terminal region (ARP38472_P050)

Catalog#: ARP38472_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Human, Pig, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-11109 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FGD1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Human: 100%; Pig: 86%; Rat: 79%
• Peptide Sequence: Synthetic peptide located within the following region: HGHRAPGGAGPSEPEHPATNPPGAAPPACADSDPGASEPGLLARRGSGSA
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-FGD1 (ARP38472_P050) antibody is Catalog # AAP38472 (Previous Catalog # AAPP23401)
• Target Reference: Bottani,A., (2007) Am. J. Med. Genet. A 143A (19), 2334-2338
• Publications:

  Zou, W. et al. MLK3 regulates bone development downstream of the faciogenital dysplasia protein FGD1 in mice. J. Clin. Invest. 121, 4383-92 (2011). WB, Cow, Human, Pig, Rat 21965325

• Gene Symbol: FGD1
• Official Gene Full Name: FYVE, RhoGEF and PH domain containing 1
• Alias Symbols: AAS, FGDY, ZFYVE3, MRXS16
• NCBI Gene Id: 2245
• Protein Name: FYVE, RhoGEF and PH domain-containing protein 1
• Description of Target: FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome.FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Swissprot Id: P98174
• Protein Accession #: NP_004454
• Nucleotide Accession #: NM_004463
• Protein Size (# AA): 961
• Molecular Weight: 106kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express FGD1.
• Protein Interactions: BTRC; UBC; ELAVL1; ELMO1; CTTN; CDC42; AOC1;

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

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Product Protocols: FGD1 antibody tested with Human Transfected 293T Cells (ARP38472_P050)