FGA ELISA Kit (Human) : 96 Wells (OKEH02742)

Catalog No: OKEH02742

Size:96 Wells

Human FGA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FGA ELISA Kit (Human) (OKEH02742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGA captured in well.
• ELISA Kit Range: 0.78 - 50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.5% (n = 20)
  Mean Inter-assay CV%: < 6.7% (n = 20)
• ELISA Kit Component:
  • Anti-FGA Microplate: 96 Wells (12 x 8 Well strips)
  • FGA Lyophilized Standard: 2
  • 100X Biotinylated FGA Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.22 ng/mL
• Specificity: Natural and recombinant Human Fibrinogen alpha chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGA
• Gene Full Name: fibrinogen alpha chain
• Alias Symbols: Fib2
• NCBI Gene Id: 2243
• Protein Name: Fibrinogen alpha chain
• Description of Target: This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing.
• Uniprot ID: P02671
• Protein Accession #: NP_000499.1
• Nucleotide Accession #: NM_000508.4
• Protein Size (# AA): 866
• Molecular Weight: 95 kDa