FGA ELISA Kit (Bovine) (OKEH03913)

Catalog No: OKEH03913

Size:96 Wells

Bovine FGA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bovine
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FGA ELISA Kit (Bovine) (OKEH03913) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGA captured in well.
• ELISA Kit Range: 7.8 - 500ng/mL
• ELISA Kit Component:
  • FGA Microplate: 96 Wells (12 x 8 Well strips)
  • FGA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated FGA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3 ng/mL
• Specificity: Natural and recombinant Bovine Fibrinogen alpha chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGA
• Gene Full Name: fibrinogen alpha chain
• Alias Symbols: Fibrinogen alpha chain
• NCBI Gene Id: 522039
• Protein Name: Fibrinogen alpha chain
• Description of Target: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
• Uniprot ID: P02672
• Protein Accession #: NP_001028798.1
• Nucleotide Accession #: NM_001033626.1
• Protein Size (# AA): 615
• Molecular Weight: 615 kDa