FFAR4 ELISA Kit (Rat) (OKEH06703)

Catalog No: OKEH06703

Size:96T

Rat FFAR4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ffar4 ELISA Kit (Rat) (OKEH06703) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ffar4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ffar4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ffar4 captured in the well.
• ELISA Kit Range: 0.625-40ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into serum = 99%
• ELISA Kit Reproducibility: Intra-Assay Reproducibility CV% < 5.4% (n=20)< br>Inter-Assay Reproducibility CV% < 8.5% (n=24)
• ELISA Kit Component:
  • NF1 Microplate: 96 Wells (12 x 8 Well strips)
  • NF1 Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated NF1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.625 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ffar4
• Gene Full Name: free fatty acid receptor 4
• Alias Symbols: free fatty acid receptor 4;Gpr120;G-protein coupled receptor 120;O3far1;omega-3 fatty acid receptor 1.
• NCBI Gene Id: 294075
• Protein Name: Q2AC31
• Description of Target: Free fatty acid receptor 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q2AC31
• Protein Accession #: NP_001040553
• Nucleotide Accession #: NM_001047088