FFAR1 Antibody (OAAF04881)

Catalog No: OAAF04881 (Formerly GWB-ASF280)

Size:100 ug

Western blot analysis of extracts from COS7 cells, treated with forskolin 40nM 30', using FFAR1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of LOVO cells, using FFAR1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Monkey
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FFAR1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: FFAR1antibodydetects endogenous levels of totalFFAR1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:10000
• Reference: Sawzdargo M., Biochem. Biophys. Res. Commun. 239:543-547(1997).
  The MGC Project Team; Genome Res. 14:2121-2127(2004).
  Briscoe C.P., J. Biol. Chem. 278:11303-11311(2003).

Target Info

• Gene Symbol: FFAR1
• Gene Full Name: free fatty acid receptor 1
• Alias Symbols: FFA1R;free fatty acid receptor 1;GPCR40;GPR40;G-protein coupled receptor 40.
• NCBI Gene Id: 2864
• Protein Name: Free fatty acid receptor 1
• Description of Target: G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity). Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.
• Uniprot ID: O14842
• Protein Accession #: NP_005294.1
• Molecular Weight: 31 kDa