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Catalog No: OKCD01608 (Formerly GWB-KBBNU1)
Size:96WELLS
Price: $750.00
SKU
OKCD01608
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Datasheets/ManualsPrintable datasheet for FETUB ELISA Kit (Bovine) (OKCD01608)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)98-105%93-102%81-89%85-101%
EDTA Plasma (n=5)90-98%95-102%87-95%79-98%
Heparin Plasma (n=5)94-102%82-90%79-92%78-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fetuin B (FETUB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fetuin B (FETUB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fetuin B (FETUB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fetuin B (FETUB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)99-105102
EDTA Plasma (n=5)90-9994
Heparin Plasma (n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fetuin B (FETUB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fetuin B (FETUB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-FETUB Microplate96 Wells (12 x 8 Well Strips)
FETUB Lyophilized Standard2 x 2,000 ng
100X Biotinylated FETUB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 11.7 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fetuin B (FETUB). No significant cross-reactivity or interference between Fetuin B (FETUB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFETUB
Gene Full Namefetuin B
Alias Symbolsfetuin-B.
NCBI Gene Id504615
Protein NameFetuin-B
Description of TargetProtease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity).
Uniprot IDQ58D62
Protein Accession #NP_001029390.1
Nucleotide Accession #NM_001034218.2
Protein Size (# AA)387
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