FEN1 Protein (OPPA02024)

Catalog No: OPPA02024 (Formerly GWB-C35B67)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid.20mM Tris-HCl buffer pH-8.0, 1mM DTT, 10% glycerol.
  Physical Appearance: Sterile filtered colorless solution.
• Reconstitution and Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
• Purification: The FEN1 protein is purified by standard chromatogrpahy techniques.
• Concentration: Varies by lot. See vial for concentration.
• Purity: > 90.0% as determined by SDS-PAGE.
• Protein Sequence: MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK.
• Source: E. coli

Target Info

• Gene Symbol: FEN1
• Alias Symbols: MF1, RAD2, FEN-1
• NCBI Gene Id: 2237
• Protein Name: Flap endonuclease 1
• Description of Target: FEN1 removes 5'' overhanging flaps in DNA repair and processes the 5'' ends of Okazaki fragments in lagging strand DNA synthesis. The interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, therefore passing the substrate from one enzyme to another. FEN1 is part of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5'' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
• Uniprot ID: P39748
• Protein Accession #: NP_004102.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 43 kDa