Fen1 ELISA Kit (Mouse) (OKEH08214)

Catalog No: OKEH08214

Size:96T

Mouse Fen1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology Fen1 ELISA Kit (Mouse) (OKEH08214) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fen1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fen1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fen1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.6% (n = 20)
  Mean Inter-assay CV%: <=7.2% (n = 20)
• ELISA Kit Component:
  • Fen1 Microplate: 96 Wells (12 x 8 Well strips)
  • Fen1 Lyophilized Standard: 2
  • 100X Biotinylated Fen1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  Subcellular Location: Nucleus Nucleolus Nucleus Nucleoplasm Mitochondrion Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  Unigene: Mm.2952
  String: 10090.ENSMUSP00000025651
  Pfam: PF00867
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.27ng/mL
• Specificity: Natural and recombinant Mouse Flap endonuclease 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fen1
• Alias Symbols: Flap structure-specific endonuclease 1.
• Protein Name: P39749
• Description of Target: Flap endonuclease 1
• Uniprot ID: https://www.uniprot.org/uniprot/P39749