Catalog No: OKEH08213
Size:96T
Price: $725.00
SKU
OKEH08213
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology FEN1 ELISA Kit (OKEH08213) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-FEN1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FEN1 and incubated. The FEN1 found in the sample or standards competes with the biotinylated FEN1 for limited binding sites on the immobilized anti-FEN1 antibody. Excess unbound biotinylated FEN1 and sample or standard FEN1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FEN1 captured in the well and inversely proportional to the amount of FEN1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=4.2% (n = 20) Mean Inter-assay CV%: <=7.0% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. | ||||||||||||||||||||||
:: | Subcellular Location: Isoform FENMIT Mitochondrion | ||||||||||||||||||||||
:: | Unigene: Hs.409065 SMR: P39748 String: 9606.ENSP00000305480 | ||||||||||||||||||||||
:: | KEGG: hsa:2237 MIM: 600393 Pfam: PF00867 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.136ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Flap endonuclease 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | FEN1 |
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Gene Full Name | flap structure-specific endonuclease 1 |
Alias Symbols | DNase IV;FEN-1;flap endonuclease 1;Flap structure-specific endonuclease 1;Maturation factor 1;maturation factor-1;MF1;RAD2. |
NCBI Gene Id | 2237 |
Protein Name | P39748 |
Description of Target | Flap endonuclease 1 |
Uniprot ID | https://www.uniprot.org/uniprot/P39748 |
Protein Accession # | NP_004102.1 |
Nucleotide Accession # | NM_004111.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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