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Catalog No: OKEH08213
Size:96T
Price: $725.00
SKU
OKEH08213
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)91-101% (n = 20)104-114% (n = 20)81-93% (n = 20)101-114% (n = 20)
EDTA plasma(n=5)93-102% (n = 20)103-112% (n = 20)92-104% (n = 20)105-115% (n = 20)
heparin plasma(n=5)80-90% (n = 20)97-107% (n = 20)111-121% (n = 20)84-94% (n = 20)
ELISA Kit PrincipleAviva Systems Biology FEN1 ELISA Kit (OKEH08213) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-FEN1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FEN1 and incubated. The FEN1 found in the sample or standards competes with the biotinylated FEN1 for limited binding sites on the immobilized anti-FEN1 antibody. Excess unbound biotinylated FEN1 and sample or standard FEN1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FEN1 captured in the well and inversely proportional to the amount of FEN1 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.2% (n = 20)
Mean Inter-assay CV%: <=7.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-FEN1 Microplate96 Wells (12 x 8 Well strips)
FEN1 Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X FEN1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
::Subcellular Location: Isoform FENMIT Mitochondrion
::Unigene: Hs.409065
SMR: P39748
String: 9606.ENSP00000305480
::KEGG: hsa:2237
MIM: 600393
Pfam: PF00867
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.136ng/mL
SpecificityNatural and recombinant Human Flap endonuclease 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolFEN1
Gene Full Nameflap structure-specific endonuclease 1
Alias SymbolsDNase IV;FEN-1;flap endonuclease 1;Flap structure-specific endonuclease 1;Maturation factor 1;maturation factor-1;MF1;RAD2.
NCBI Gene Id2237
Protein NameP39748
Description of TargetFlap endonuclease 1
Uniprot IDhttps://www.uniprot.org/uniprot/P39748
Protein Accession #NP_004102.1
Nucleotide Accession #NM_004111.5
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