FEN1 Antibody (OAGA08755)

Catalog No: OAGA08755

Size:100 ul

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7] antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 4E7
• Isotype: IgG1
• Host: Mouse
• Application: ICC, IF, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
• Immunogen: Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
• Purification: Protein G purified
• Concentration: 1.73 mg/ml
• Application Info: Suggested starting dilutions are as follows: ICC/IF, IP, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: FEN1
• Gene Full Name: flap structure-specific endonuclease 1
• Alias Symbols: MF1, RAD2, FEN-1
• NCBI Gene Id: 2237
• Protein Name: flap endonuclease 1
• Description of Target: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
• Uniprot ID: P39748
• Protein Accession #: NP_004102.1
• Nucleotide Accession #: NM_004111.5