Catalog No: OCOA00048
Size:20UG
Price: $179.00
SKU
OCOA00048
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

FECH Protein Lysate (OCOA00048)

Datasheets/ManualsPrintable datasheet for FECH Protein Lysate (OCOA00048)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFECH HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFECH
Gene Full Nameferrochelatase
Alias SymbolsEPP;EPP1;FCE;ferrochelatase, mitochondrial;heme synthase;heme synthetase;protoheme ferro-lyase;protoporphyria.
NCBI Gene Id2235
Protein NameNM_000140, NP_000131, P22830
Description of TargetFerrochelatase, mitochondrial, ferrochelatase, mitochondrial isoform b precursor [Homo sapiens], Homo sapiens ferrochelatase (FECH), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P22830
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000131
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000140
Molecular Weight47.9 kDa
Write Your Own Review
You're reviewing:FECH Protein Lysate (OCOA00048)
Your Rating