FDXR ELISA Kit (Rat) (OKEH08332)

Catalog No: OKEH08332

Size:96T

Rat FDXR ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FDXR ELISA Kit (Rat) (OKEH08332) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FDXR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FDXR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FDXR captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.4% (n = 20)
  Mean Inter-assay CV%: <=7.7% (n = 20)
• ELISA Kit Component:
  • FDXR Microplate: 96 Wells (12 x 8 Well strips)
  • FDXR Lyophilized Standard: 2
  • 100X Biotinylated FDXR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.
  Subcellular Location: Mitochondrion matrix
  Unigene: Rn.10860
  SMR: P56522
  String: 10116.ENSRNOP00000004592
  KEGG: rno:79122
  Pfam: PF07992
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.12ng/mL
• Specificity: Natural and recombinant Rat NADPH:adrenodoxin oxidoreductase, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FDXR
• Gene Full Name: ferredoxin reductase
• Alias Symbols: AR
• NCBI Gene Id: 79122
• Protein Name: NADPH:adrenodoxin oxidoreductase, mitochondrial
• Description of Target: component of electron transfer system for mitochondrial cytochromes P-450; mediates production of steroid hormones and bile acids; activates vitamin D3 in the steroidogenic tissues, liver and kidney [RGD, Feb 2006]
• Uniprot ID: P56522
• Protein Accession #: NP_077067.1
• Nucleotide Accession #: NM_024153.1