Catalog No: OKCD08610
Size:96WELLS
Price: $850.00
SKU
OKCD08610
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Datasheets/ManualsPrintable datasheet for FDPS ELISA Kit (Rat) (OKCD08610)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyl Diphosphate Synthase (FDPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyl Diphosphate Synthase (FDPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyl Diphosphate Synthase (FDPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Farnesyl Diphosphate Synthase (FDPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FDPS Microplate96 Wells (12 x 8 Well strips)
FDPS Lyophilized Standard2
100X FDPS HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.057ng/mL
Gene SymbolFdps
Gene Full Namefarnesyl diphosphate synthase
Alias Symbols(2E,6E)-farnesyl diphosphate synthase;Ac2-125;cholesterol-regulated 39 kDa protein;CR 39;dimethylallyltranstransferase;Farnesyl diphosphate synthase;farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase);farnesyl diphosphate synthetase;farnesyl pyrophosphate synthase;farnesyl pyrophosphate synthetase;FPP synthase;FPP synthetase;FPS;geranyltranstransferase;testis-specific farnesyl pyrophosphate synthetase.
NCBI Gene Id83791
Protein NameFarnesyl pyrophosphate synthase
Description of TargetKey enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate (By similarity).
Uniprot IDP05369
Protein Accession #NP_114028.1
Nucleotide Accession #NM_031840.1
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