FCN3 ELISA Kit (Human) (OKCD07780)

Catalog No: OKCD07780

Size:96WELLS

FCN3 ELISA Kit (Human) (OKCD07780) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ficolin 3 (FCN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ficolin 3 (FCN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ficolin 3 (FCN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ficolin 3 (FCN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 3 (FCN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 3 (FCN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FCN3 Microplate: 96 Wells (12 x 8 Well strips)
  • FCN3 Lyophilized Standard: 2
  • 100X FCN3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 37pg/mL

Target Info

• Gene Symbol: FCN3
• Gene Full Name: ficolin 3
• Alias Symbols: collagen/fibrinogen domain-containing lectin 3 p35;collagen/fibrinogen domain-containing protein 3;FCNH;ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen);ficolin-3;HAKA1;Hakata antigen;H-ficolin.
• NCBI Gene Id: 8547
• Protein Name: Ficolin-3
• Description of Target: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.
• Uniprot ID: O75636
• Protein Accession #: NP_003656.2
• Nucleotide Accession #: NM_003665.3